miR-1224, located at chromosome 3q27.1, is a class of mammalian mirtron encompassed in the last intron of the VWA5B2 gene (von Willebrand factor A domain containing 5B2) and is discovered that acts vital roles in some diseases, such as acute liver failure, Parkinson’s disease, and cerebral ischemia (13–15). Here, VWA5B2 is linked to Cerebral ischemia.