In order to investigate the impact of TIM-3 expression of leukemic blasts on lymphocyte subtypes, we assessed the proportions of lymphocyte subtypes (CD8+ T cells, CD4+ T cells, CD4+ Treg cells, B cells and NK cells) and concentrations of helper T cell-1 (Th1)/Th2/Th17 cytokines (tumor necrosis factor-α, interferon-γ, interleukin-2 [IL-2], IL-4, IL-6, IL-10 and IL-17) in the peripheral blood of these AML patients. Here, IL4 is linked to acute myeloid leukemia.