The colocalization results of double immunofluorescence for TLR4 and the microglial marker IBA-1 were as follows: double immunofluorescence revealed colocalization of the microglial markers IBA-1 and TLR4 in the control group, epilepsy (EP) group, epilepsy+BAY 11–7082 (EP+BAY) group, and epilepsy+TAK242 (EP+ TAK) group. Here, CDK9 is linked to epilepsy.