Fourth, PRMT5 directly co-localized and interacted with AKT, albeit not with PTEN and mTOR; Akt phosphorylation at Thr308 and Ser473 and the downstream target GSK3 at Ser9 was markedly decreased without altering PTEN and mTOR phosphorylation at Ser2442 in PRMT5-deficient lung adenocarcinoma cells (183). This evidence concerns the gene PRMT5 and lung adenocarcinoma.