Multiple subtypes of breast cancer were selected as research subjects in this study, including four invasive subtypes (Luminal A, Luminal B, HER-2 enriched, and Triple Negative Breast Cancer (TNBC) cases previously classified by immunohistochemical staining) and Ductal Carcinoma in situ (DCIS) cases (Table 1). This evidence concerns the gene ERBB2 and ductal breast carcinoma in situ.