Additionally, EVs purified from MC38 colon carcinoma cells overexpressing IL-12 and deprived of TGF-β1 by transfecting shRNA molecules targeting TGFB1, efficiently inhibited tumor growth and induced anti-tumor immunity, together with DC-based vaccines (Rossowska et al., 2019). This evidence concerns the gene TGFB1 and colon carcinoma.