To fully clarify the role of VSIR in the TME in pan-cancer, we used single-cell sequencing analysis to observe the co-expression of VSIR with tumor and inflammatory cells in BLCA (Figure 6A), CHOL (Figure 6B), HNSC (Figure 6C), KIRC (Figure 6D), LUAD (Figure 6E), GBM (Figure 7A), BRCA (Supplementary Figure S11A), COAD (Supplementary Figure S11B), LIHC (Supplementary Figure S11C), and STAD (Supplementary Figure S11D). The gene discussed is VSIR; the disease is cancer.