The cell cycle detection revealed that in circ-ASB3 down-regulated glioma cells, the percentage of cells in G0/G1 phase decreased and percentage of cells in S phase increased, while in glioma cells combined with miR-543 inhibition or Twist1 overexpression, the percentage of cells in G0/G1 phase correspondingly increased and percentage of cells in S phase correspondingly decreased (Figures 5E,F). This evidence concerns the gene TWIST1 and central nervous system cancer.