To validate this interaction in vivo, we performed confocal co-localization studies of Nras immunofluorescence, mCherry autofluorescence as an indicator of an effective infection by sgR-equipped rAAV and GFP autofluorescence as a label of POMCCreCas9-expressing neurons in POMCCremiR-29aCKO mice. The gene discussed is NRAS; the disease is infection.