Indeed, POMCCremiR-29CKO mice revealed early onset food intake increase, late onset obesity, hypertrophy of adipocytes, and increased insulin resistance (Figure 6D, Figure S5) while simultaneous deletion of Nras in the same type of neurons of POMCCremiR-29CKO + NrasCKO mice significantly attenuated this phenotype indicating the contribution of Nras up-regulation to POMCCremiR-29CKO phenotype. Here, NRAS is linked to obesity due to melanocortin 4 receptor deficiency.