To prove that Nras plays a role in the obesity phenotype of POMCCremiR-29CKO mice, we designed (Figure 6B, Table S1, File S1), validated (Figure 6C) and packaged Nras-targeting sgR-n1 and -n2 into rAAV (File S2). This evidence concerns the gene NRAS and obesity due to melanocortin 4 receptor deficiency.