Determining CD19 expression in the aggressive lymphoma subtypes diffuse large B cell lymphoma (DLBCL, Fig. 2B) and mantle cell lymphoma (MCL, Fig. 2C) we again found that use of a low affinity CD19 CAR significantly reduced CD19 loss on the tumor cells (Fig. 2D, E), demonstrating that affinity modulation represents an effective strategy to reduce trogocytosis across B cell malignancies. This evidence concerns the gene CD19 and diffuse large B-cell lymphoma.