Dynamic monitoring of immune microenvironment revealed significantly elevated numbers of CD8+ T cells and an increased ratio of CD8+ to CD4+ T cells (Fig. 6d), particularly antigen-specific tumor-reactive T cells (Fig. 6e, f), in which the level of circulatory IFN-γ significantly rose and TGF-β declined (Fig. 6g) in mice treated with DEXP&A&N in combination with Flt3L compared to PBS controls on day 42, indicating a remodeling of immune microenvironment. The gene discussed is TGFB1; the disease is neoplasm.