As Batf3 is an important transcription factor required for development of cross-presenting CD103+CD11c+ and CD8α+CD11c+ DCs in mice [29, 30], to verify the role of cross-presenting DCs in the functionality of DEXP&A2&N, we intravenously administered DEXP&A2&N to orthotopic Batf3−/− HCC mice bearing OVA-expressing tumors. This evidence concerns the gene CD8A and hepatocellular carcinoma.