Subsequent subgroup analysis based on the absence or presence of lymph node metastasis showed that in BUC, the pelvic lymph node metastasis was significantly associated with pathological stage, pathological grade, tumor number, tumor diameter, and NUSAP1 expression (p < 0.05, see Table 4), but not with the gender and age of the patients (p > 0.05, see Table 4). The gene discussed is NUSAP1; the disease is neoplasm.