TP53 and cancer: Across all non-cancer screening samples (n = 61), two likely pathogenic SNV variants (JAK2 V617F, detected at 0.81% VAF, and TP53 M246I, detected at 0.40% VAF) were observed, while no INDELs, CNAs, gene fusions, or non-genomic biomarkers, were detected, giving a per-sample clinical specificity of 96.72%, or per-base specificity of ≥99.9999% (2 SNVs across 3.59 million bp, or 61 samples x 58.8 kb per sample).