WT1 and acute myeloid leukemia: In this sense, with or without expression of the WT1 antigen, we could detect residual leukemic cells in AML-surviving mouse PB and BM by flow cytometry and RT-qPCR from 56 days (8 weeks) up to 126–132 days (17–18 weeks) post-Ara-c treatment, mostly confirming their persistence.