Following the previously described optimization of the Click Beads with DSPE‐PEG1000‐TCO protocol, we then explored its clinical utility in Ewing sarcoma with an modified workflow to quantify the two most common subtypes of EWS gene rearrangements (i.e., EWS/FLI‐1 and EWS/ERG, Figure S4 and Table S2, Supporting Information) in Ewing sarcoma patients (Figure4A). The gene discussed is EWSR1; the disease is Ewing sarcoma.