Compared with CD133−CD44− colon cancer cells, the intracellular concentrations of total iron and Fe2+ (Figure 9(A)), mitochondrial superoxide (Figure 9(B)) and lipid ROS (Figure 9(C)) were substantially higher than those in CD133−CD44− cells, whereas the mitochondrial membrane potential was significantly lower (Figure 8(D)), indicating that CD44+CD133+ colon CSCs blocked the process of ferroptotic cell death. This evidence concerns the gene PROM1 and colonic neoplasm.