Using the in vivo limiting dilution assay, we found that EHD1 overexpression enhanced the efficiency of tumour generation or tumoursphere formation and enlarged the final xenograft size in nude mice (Figure 4F), whereas treatment with VP decreased the EHD1‐induced enhancement of tumoursphere formation efficiency and reversed cancer cell stemness (Figure 4G). This evidence concerns the gene EHD1 and neoplasm.