As expected, overexpression EHD1 enhanced the CSCs properties of LUAD cells, as further indicated by the 3D spheroid cancer models (Figure S2C and D), the holoclone assay (Figure S2E), stem cell marker evaluation (Figure S2F), flow cytometric analysis of CD133‐ and CD44‐positive cells (Figure S2G), and limiting dilution assay (Figure S2H). This evidence concerns the gene CD44 and cancer.