To test the effect of BXL4 overexpression on the induction of PDF1.2 and PAD3 expression, the inducible BXL4 overexpression lines were sprayed with β-estradiol or mock-induced by spraying 0.01% Tween 20, and 4 days later the plants were infected with B. cinerea. Mock treatment did not result in any induction of PDF1.2 or PAD3 prior to infection (Figure 6, A and C, time-point 0 h). This evidence concerns the gene PADI3 and infection.