Analysis of the transcriptome change occurring in the NASH-induced spheroids revealed that expression of FGF21 (lipid metabolism), FXR (oxidative stress), CHOP (ER stress and mitochondrial imbalance) and CXCL5 (inflammation) drastically increased in order to cope with the altered metabolic, redox and energy balance [32,37,38,66–68]. Here, DDIT3 is linked to metabolic dysfunction-associated steatohepatitis.