For tumor-derived organoids, we picked cells from three individual organoids (Org-1, Org-2, Org-3, Additional file 1: Fig. S3A) and one batch of organoid mixtures (Mix, Additional file 1: Fig. S3B), some of the cells from Mix and all cells from Org-1 did not have tumor-specific mutations, whereas the majority of the cells from Org-2, Org-3 have tumor-specific mutations (Fig. 4B). The gene discussed is MIXL1; the disease is neoplasm.