PFKFB4 and primary ovarian failure: On BTA22, there were five suggestive candidate genes, including the transforming growth factor beta receptor 2 (TGFBR2) for female fertility in pigs and mice [62, 63], DLEC1 cilia and flagella associated protein (DLEC1) and solute carrier family 22 member 14 (SLC22A14) regulating male fertility in mice [64, 65], activin A receptor type 2B (ACVR2B) associated with premature ovarian failure in human [66], and 6-phosphofructo-2-kinase fructose-2,6-biphosphatase 4 (PFKFB4) involved in spermatogenesis in mice [67].