Combining recombinase polymerase amplification (RPA) technology with collateral ssDNase activity of Cas13, utilized in this method, is used for highly sensitive and specific detection of mutations associated with cancer in cfDNA, such as EGFR-T790M and EGFR-L858R mutations in patients with non-small-cell lung carcinoma (NSCLC) [98]. This evidence concerns the gene EGFR and cancer.