Moreover, a markedly higher degree of heterogeneity in NECTIN4 protein expression was observed in multifocal TaHG tumors from mixed-grade lesions (heterogenous overall NECTIN4 staining: 22%, heterogenous membranous NECTIN4 staining: 33%) in comparison to multifocal CIS/T1HG (heterogenous overall staining: 9%, heterogenous membranous staining: 2%) and pure TaHG carcinomas (heterogenous overall staining: 5%, heterogenous membranous staining: 9%) (Table 3). This evidence concerns the gene NECTIN4 and in situ carcinoma.