CD40LG and Alzheimer disease: To investigate the levels of IgM and IgG in different stages of AD brain tissue, supernatants produced from homogenized cortical gray matter were subjected to western blot analysis using anti-human antibodies for heavy chains Gamma (IgG-γ, Kruskal–Wallis test: p = 0.039) and Mu (IgM-μ, Kruskal–Wallis test: p = 0.006) (Fig. 1).