In organotypic bronchial epithelial cultures from 14 children and 10 older adults, replication of SARS-CoV-2 WA-01 was compared between cultures infected with SARS-CoV-2 alone (MOI = 0.5), infection of cultures with HRV-16 (MOI = 0.5) followed 72 h later by infection with SARS-CoV-2 (MOI = 0.5), infection of IFNβ1 pre- and concurrently treated cultures with SARS-CoV-2, and infection of IFNλ2 pre- and concurrently treated cultures with SARS-CoV-2. This evidence concerns the gene IFNB1 and infection.