Through cell experiment verification, the levels of GSEC, SNX16, and PAPOLG mRNAs were highly expressed in SMMC-7721, HUH-7, MHCC97-H, and SNU449 HCC cell lines, while the expression level of miR-101-3p was significantly lower in those HCC cell lines, which is consistent in HCC patient samples through bioinformatics analysis. The gene discussed is PAPOLG; the disease is hepatocellular carcinoma.