Additionally, top hub genes included TMEM258, KRTCAP2, MTX1, SCAMP3, CCT3, RFWD2, PSMB4, CACYBP, and PFDN2, etc. ROC was also used to analyse the distinguishing efficacy of SSR2 between HCC and normal liver tissues. The gene discussed is TMEM258; the disease is hepatocellular carcinoma.