Similarly, we demonstrated that compared with the lipofectamine 6000-mediated siWISP1 transfection, the expression of WISP1 in breast cancer cells was further decreased after US-mediated delivery of siWISP1, and meanwhile the cell migration, invasion, and proliferation were further inhibited, indicating that US could enhance the transfection efficiency and promote the antitumor activity of siWISP1. The gene discussed is CCN4; the disease is breast carcinoma.