SIRT6 and metabolic disease: However, in this study we only synthesized the 5′tsRNA-Gly-GCC that had been found increased in F0 sperm for the experiment to study whether the 5′tsRNA-Gly-GCC could target Sirt6 and induced the metabolic disorder in hepal-6 cells, and we did not test whether the synthesized tsRNAs without modification could transfer the paternal acquired phenotype to the offspring.