However, a signature pattern of epigenetic regulation of PRSS3 expression by site-specific CpG methylation was dynamically shown from mPRSS3Low to umPRSS3High through clinical progression, better matched to PRSS3-V2, suggesting PRSS3-V2 to be a more prevalent isoform functionally through clinical progression of HCC. Here, PRSS3 is linked to hepatocellular carcinoma.