PRSS3 and hepatocellular carcinoma: Association analysis demonstrated an inverse association between the upstream methylation and mRNA expression of PRSS3 and its transcripts PRSS3-V1 and -V2 that could distinguish HCC cell lines phenotypically between hypermethylation of PRSS3Low (mPRSS3Low) and hypomethylation of PRSS3High (umPRSS3High) groups (Figure 3B).