Since the region located at site D was shown to be an important regulatory region specifically for epigenetic regulation of PRSS3 transcripts (Figure 3), the data suggest that site-specific epigenetic alteration of PRSS3-V2 in HCC tissues was distinct between mPRSS3-V2Low in early HCC and umPRSS3High in advanced HCC patients, in which early HCC patients with PRSS3-V2Low tumors had better outcomes. This evidence concerns the gene PRSS3 and hepatocellular carcinoma.