Analysis of the DepMap data revealed that in 24 HCC cell lines, PRSS3-V2 and/or -V1 were two major transcripts contributing to the expression of PRSS3 because PRSS3-V3 was infrequent and/or poorly expressed, while PRSS3-V4 was absent (Table S1 and Figure 2B). This evidence concerns the gene PRSS3 and hepatocellular carcinoma.