Moreover, the results of the clone formation assay showed that overexpression of PRSS3-V1 or PRSS3-V3 remarkably diminished the number of colonies of HCC cells compared with the control group, but PRSS3-V2 overexpression resulted in an increased number of colonies only effectively in HepG2 cells. The gene discussed is PRSS3; the disease is hepatocellular carcinoma.