PRSS3 transcript heterogeneity was further evidenced by its divergent responses to 5-aza-CR treatment of HCC cells, distinguishing between upregulation of PRSS3-V1 or -V3 but downregulation of PRSS3-V2 in PRSS3Low HCC and downregulation of PRSS3-V1 but upregulation of PRSS3-V2 in PRSS3High HCC. Here, PRSS3 is linked to hepatocellular carcinoma.