CD63 and monocytic leukemia: Recently, we developed and characterized a human monocytic leukemia THP-1 reporter cell line engineered with a fusion construct for the expression of EV-associated tetraspanins (CD63 and CD9) linked to Turbo-luciferase (Tluc) and Emerald Green Fluorescent Protein (EmGFP) (CD63 Tluc-CD9-EmGFP THP-1 cells) to quantitatively measure release of EVs in culture supernatants (Shpigelman et al., 2021).