In conclusion, this manuscript provided comprehensive analyses about the expression and function of MYOs in HNSCC, suggested MYO1B, MYO5A, and MYO10 as potential prognostic biomarkers in HNSCC, and revealed the potential novel roles of MYO1B, MYO5A, and MYO10 in tumor immune microenvironment. Here, GOSR2 is linked to head and neck squamous cell carcinoma.