ASCL1 and glioblastoma: ASCL1 bound regions corresponded largely to enhancer (intronic and intergenic) regions (Fig. 4c), consistent with previous reports in glioblastoma and normal neurons25,26.We identified 3,205 ASCL1 bound genes common between the two cell lines and as expected, pathway analysis identified ASCL1 bound genes to be involved in stem and neuronal programming in NEPC cell lines (Fig. 4d).