ASCL1 and adenocarcinoma: In agreement with changes in the chromatin architecture, ASCL1 expression rapidly increased upon ENZ treatment of adenocarcinoma cell lines in a time-dependent manner (Fig. 2d), in LNCaP cell lines following androgen deprivation (Supplementary Fig. 2e), and in patient derived xenografts (PDX) models post castration19 (Supplementary Fig. 2f).