Importantly, and in line with the discovery findings, stratification of the HiFi tumours in this independent validation cohort using the median of HPS (again closely aligned to AUROC optimal cut-off; online supplemental figure 3), revealed that those with a low expression (n=26) had significantly lower IFN alpha and IFN gamma response signalling (figure 2F) and poorer RFS compared with those with a high expression (n=26) (relapse rates of 46.2% and 7.7%, respectively; figure 2G; top; log-rank p=0.00113). The gene discussed is IFNG; the disease is neoplasm.