Interrogation of single-cell RNA-Seq data from tumour-infiltrating immune populations isolated from a further independent cohort of CRC tumours,32 which again confirmed a significant elevation of HPS expression (figure 4E; t-test p<0.0001) and APP signalling (figure 4F; t-test all p<0.0001) in tumour infiltrating monocytes, macrophages and to a greater extent in DCs compared with epithelial and CAF populations. Here, APP is linked to colorectal carcinoma.