As shown in Fig. 2, (panel A, C and E indicates infected CRC cells with HSV-HMGB1(red) at MOI 0.7, and panel B, D and F indicates CRC cells stained with anti HMGB1 antibody (green)) based on fluorescence signal per cell, the mean basal expression of HMGB1 protein in uninfected HCT116 (mean ± SD, 21.25 ± 2.3 AU), SW480 (mean ± SD, 4.62 ± 1.21 AU) and HT29 cells (mean ± SD, 12 ± 1.95 AU) were significantly lower when compared to infected HCT116 (mean ± SD, 43.37 ± 2.1 AU), SW480 (mean ± SD, 23.5 ± 1.21 AU) and HT29 cells (mean ± SD, 29.1 ± 1.54 AU) (Fig. 2 panel G, and Table 1). Here, HMGB1 is linked to colorectal carcinoma.