HMGB1 and infection: As seen in Fig. 4, and Table 2, in HCT116 cells, Annexin/PI double staining revealed that the apoptosis following HSV-HMGB1 infection has increased to 54.3% and 49.1%, that were nearly 2.8 and 1.8 times more than that of HSV-ble 19% and 26.7% during normoxia and hypoxia, respectively.