Therefore, to investigate whether autophagy plays a part in reducing the HSV-HMGB1 cytotoxicity in hypoxic conditioned HT29 cells, we examined the level of LC3-II expression in HCT116, SW480, and HT29 after infection with HSV- HMGB1 and HSV-ble during hypoxia and normoxia. Here, HMGB1 is linked to infection.