Here, to determine whether oncolytic virotherapy and overexpression of HMGB1 under hypoxic conditions can affect subcellular localization of the protein and tumor cell death, we infected three colorectal cancer cell lines with a recombinant oHSV expressing HMGB1 and investigated its effects on the cell lines in in vitro normoxic and hypoxic environments. The gene discussed is HMGB1; the disease is neoplasm.