WASF2 and hepatocellular carcinoma: We performed BSP and MSP analysis on the WASF2 promoter in immortalized normal liver hepatocytes (THLE-2 and MIHA) and HCC (Hep3B,Huh-7 and SNU475) cell lines targeting the region including the cg24162579 site (− 833 to − 534 bp relative to the transcriptional start site, Fig. 5A).