To observe the dynamics of NEMO puncta in the cytoplasm, we performed live cell imaging of NEMO and TRAF6 in the human colorectal carcinoma cell line HCT116 in which endogenous NEMO was knocked out by CRISPR and reconstituted with mCherry-tagged NEMO and green fluorescent protein (GFP) tagged TRAF6. The gene discussed is IKBKG; the disease is colorectal carcinoma.