There were significant differences in IL-8, IL-10, TNF, and VEGF concentration between leukemia and head and neck carcinoma at pre-index (p < 0.05, p < 0.05, p < 0.001, and p < 0.01, respectively; Fig 6D, 6E, 6G and 6I) and IL-8, TNF, VEGF concentration were higher in the saliva of head and neck carcinoma patients than leukemia patients. The gene discussed is CXCL8; the disease is leukemia.