Briefly, CD8 T cells from pmel-1 TCR transgenic mice (PMEL T cells), which are specific for the gp100 antigen expressed by B16-F10 mouse melanoma cells (37), were activated and expanded ex vivo and then tethered with IL-12 before adoptive transfer into mice bearing B16-F10 tumors (Fig. 4A). This evidence concerns the gene PMEL and melanoma.