To determine whether ovBNSTCART neurons make inhibitory synapses onto other output neurons regulating anxiety-like behavior, electrophysiological analyses were performed using BNST slices prepared from Cart-Cre mice injected with AAV9-DIO-hSyn-ChR2-EYFP into the ovBNST and retrobeads into the VTA (fig. The gene discussed is CARTPT; the disease is Anxiety.