To test whether inhibition of LH-projecting adBNST neurons enhanced anxiety-like behavior in naïve mice, we bilaterally infused retrograde-transported adeno-associated virus (AAV) 2 encoding Cre recombinase into the LH, and AAV5 carrying a Cre-dependent hM4Di-mCherry construct into the adBNST (Fig. 3, A and B). This evidence concerns the gene PLOD1 and Anxiety.