To examine the involvement of elevated excitability of ovBNSTCART neurons in chronic pain–induced maladaptive anxiety, we performed the EPM test using a mouse model of chronic pain, prepared from Cart-Cre mice injected with AAV5-hSyn-DIO-hM4Di-mCherry or AAV5-hSyn-DIO-mCherry into the ovBNST. Here, RIC8B is linked to Anxiety.