Moreover, in their recent study on the utility of serum fibulin-3 in diagnosing osteosarcoma and in predicting the prognosis, Wang et al. used ELISA in measuring the serum levels of fibulin-3 and found that serum fibulin-3 was significantly higher in osteosarcoma patients than in the control group and was significantly associated with higher stage and lung metastases [13]. This evidence concerns the gene EFEMP1 and osteosarcoma.