There was a significant correlation between the PEs score and each of the following measurements: ULCs score (r = 0.673, P < 0.001), SPAP (r = 0.529, P < 0.001), average E/e′ ratio (r = 0.421, P < 0.001), E/A (r = 0.319, P < 0.001), severity of mitral regurgitation (r = 0.394, P < 0.001), Diastolic function classification (r = 0.364, P < 0.001), LAindex (r = 0.335, P < 0.001), RAP (r = 0.346, P < 0.001), TAPSE (r = 0.221, P < 0.05), RV (r = 0.225, P < 0.05), LVEF (r =  − 0.197, P < 0.05), age (r = 0.292, P < 0.01), NYHA class (r = 0.516, P < 0.001), NT-proBNP (r = 0.398, P < 0.001). The gene discussed is NPPB; the disease is mitral valve insufficiency.