The proportion of both TCF-1+ and TCF-1+ PD-1+ cells within the Kaede Green+ and Kaede Red+ CD8 T cell populations decreased over time (Fig. 7, C and D), which equated to a significant loss in the number of Kaede Red+ TCF-1+ and TCF-1+ PD-1+ CD8 tumor T cells between 24 and 72 h (Fig. 7, E and F). The gene discussed is CD8A; the disease is neoplasm.