To fully capture cellular heterogeneity amongst the newly entering and tumor-resident TIL populations, we isolated Kaede Green+ and Kaede Red+ TILs (CD45+CD11b−/lo) from MC38 tumors at 24 and 72 h after photoconversion (named: G24, G72, R24, R72, respectively) and analyzed their transcriptomes using scRNA-seq. This evidence concerns the gene ITGAM and neoplasm.