Notably, within Kaede Green+ Tregs at 72 h after photoconversion, the proportion of NRP-1+ LAG-3− cells was reduced and the NRP-1− cells additionally contained a LAG-3+ population (Fig. 3 D), suggesting that LAG-3 expression was induced over time in the tumor and potentially identified the Treg3 and Treg4 populations. This evidence concerns the gene LAG3 and neoplasm.