Accordingly, intra-tumoral Helios+ Tregs were enriched in CCR8+ CD39+ PD-1+ subpopulations compared to DLN and splenic Tregs (Fig 3), both in obese and lean mice, further supporting the notion that tumor progression is associated with an enhanced immunosuppressive milieu [92] but suggests that obesity is not involved in the accumulation of intratumoral Tregs. The gene discussed is CCR8; the disease is obesity due to melanocortin 4 receptor deficiency.