SPINK5 and eosinophilic esophagitis: Differential analysis by pseudo-bulk RNA-Seq revealed that genes enriched in the Differentiatedlo/hi subpopulations (e.g., FLG, SPINK5) were decreased, whereas genes enriched in less differentiated populations (e.g., TGFBR1, STAT6) were increased in active EoE (Figure 7B).