Thus, the hypothesis that RELM-β drives susceptibility to colonic inflammation in the Muc2−/− model may depend on additional factors, including diet.39 Mice fed a high-fat diet (lard as a saturated fat source) exhibit higher levels of RELM-β protein in the stool and have altered glucose intolerance and hyperlipidemia due to impaired insulin signaling.39,40 Notably, our results from the MF diet, a different type of saturated fatty acid, contradict these findings, which could suggest that different types of saturated fatty acids may influence RELM-β expression. Here, INS is linked to hyperlipidemia.