In addition, by using the IncuCyte platform [23, 24], we found that the numbers of apoptotic (Annexin V+) cells were significantly higher in wells containing IECs cocultured with CD4+ T cells from R23FR mice with colitis than in wells containing IECs alone or IECs cocultured with control FR intestinal CD4+ T cells (Fig. 5D, E). The gene discussed is ANXA5; the disease is colitis.