We isolated DAPI−CD45+CD3+CD4+CD8− cells from the ceca of R23FR mice with colitis (Day 56) and from control FR mice [11, 12] (Day 56), cocultured them with IECs, and measured cell survival using a CellTiter-Glo Luminescent Cell Viability Assay Kit. The gene discussed is CD4; the disease is colitis.