As shown by Western blot assay, the overexpression or knockout of LINC00858 in SW480 and HCT166 cells did not affect the expression of RAD21 (Fig. 2C, and Supplementary Fig. 1B), suggesting that LINC00858 did not directly regulate the expression of RAD21 to participate in the colon cancer development. Here, RAD21 is linked to malignant colon neoplasm.