In order to study the effects of LINC00858 on the tumorigenicity of colon cancer cells by regulating the RAD21/PCNP/STAT3/5 axis in vivo, we injected SW480 cells transduced with adenovirus carrying oe-NC + sh-NC, oe-LINC00858 + sh-NC, oe-LINC00858 + sh-RAD21, oe-LINC00858 + sh-PCNP into nude mice. This evidence concerns the gene STAT3 and malignant colon neoplasm.