Across myotube nuclei of both MND and non-MND lines, transcripts of the n-agrin-MuSK signaling pathway (e.g. LRP4, MuSK, Dok7, caveolin 3 (CAV3) and acetylcholine receptor alpha subunit (CHRNA1)) were found to closely mirror that of the above myogenic genes, with no obvious difference in their transcript expression across MND and control samples (Fig. 9c). This evidence concerns the gene LRP4 and mild neurocognitive disorder.