IL1RN and hydrops fetalis: Next, we performed RNA-seq for specificity comparison among dWT-, dRKA-, and dHF-VPR to activate IL1RN. As a result, the dRKA-VPR system displayed a slight improvement in activation of the endogenous gene IL1RN compared with the WT and HF (Fig. 4c), demonstrating the ability of the RKA in transcriptional activation.